KMID : 0811720180220040391
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Korean Journal of Physiology & Pharmacology 2018 Volume.22 No. 4 p.391 ~ p.398
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Anti-inflammatory and utero-relaxant effect of ¥á-bisabolol on the pregnant human uterus
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Munoz-Perez Victor Manuel
Ortiz Mario I. Ponce-Monter Hector A. Monter-Perez Vicente Barragan-Ramirez Guillermo
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Abstract
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The aim of this study was to evaluate the in vitro anti-inflammatory and utero-relaxant effect of ¥á-bisabolol on the pregnant human myometrium. Samples from the pregnant human myometrium were used in functional tests to evaluate the inhibitory effect of ¥á-bisabolol (560, 860, 1,200 and 1,860 ¥ìM) on spontaneous myometrial contractions. The intracellular cyclic adenosine monophosphate (cAMP) levels generated in response to ¥á-bisabolol in human myometrial homogenates were measured by ELISA. The anti-inflammatory effect of ¥á-bisabolol was determined through the measurement of two pro-inflammatory cytokines, tumor necrosis factor-¥á (TNF¥á) and interleukin (IL)-1¥â, and the anti-inflammatory cytokine IL-10, in pregnant human myometrial explants stimulated with lipopolysaccharide (LPS). Forskolin was used as a positive control to evaluate the cAMP and cytokine levels. ¥á-Bisabolol was found to induce a significant inhibition of spontaneous myometrial contractions at the highest concentration level (p<0.05). ¥á-Bisabolol caused a concentration-dependent decrease in myometrial cAMP levels (p<0.05) and a concentration-dependent decrease in LPS-induced TNF¥á and IL-1¥â production, while IL-10 production did not increase significantly (p>0.05). The anti-inflammatory and utero-relaxant effects induced by ¥á-bisabolol were not associated with an increase in cAMP levels in pregnant human myometrial samples. These properties place ¥á-bisabolol as a potentially safe and effective adjuvant agent in cases of preterm birth, an area of pharmacological treatment that requires urgent improvement.
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KEYWORD
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¥á-bisabolol, cAMP, Immunomodulation, Inflammation, Preterm labor
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